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Group: Bioanalytical Chemistry (BACH), Department of Chemistry, University of Oslo, Oslo, Norway

Key members: Steven Ray Wilson, Hanne Røberg-Larsen

Contact: Hanne Røberg-Larsen,


Research activities: We develop techniques for measuring biosamples. Specialties are analyzing small samples, with a focus on automated/on-line sample preparation. Our analyses are typically performed with liquid chromatography and mass spectrometry. We develop and employ very narrow separation columns for nano-scale separations, which can allow us to detect attogram-amounts of analytes. For automation, we have developed an automated filtration/filter back-flush system (AFFL) for very robust microfluidics (e.g. several thousands of analyses without SPE/column/part replacement). Using these technologies, we measure oxysterols in cells/tissues/exosomes/blood/organoids.


Our main focus is on combining separation science and organoid/organ-on-chip technology, through the Center of Excellence Hybrid Technology Hub. With regards to oxysterols, we are studying Non-Alchoholic Fatty Liver Disease, with the use of liver organoids as a disease model. We are also utilizing organoids to study the health effects of persistent organic pollutions, such as per- and polyfluoroalkyl substances (PFAS) with a focus on metabolic diseases.



In addition to oxysterol analyses, we perform targeted/untargeted metabolomics of highly polar compounds, using e.g. HILIC chromatography and NMR spectroscopy and target/untargeted proteomic of limited samples. We apply our techniques in collaborations related to e.g. cancer, in-born diseases, neuroscience, and anti-terror measures.


Key papers related to ENOR:

Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids. Kømurcu KS, Wilhelmsen I, Thorne JL, Krauss S, Wilson SR, Aizenshtadt A, Røberg-Larsen H. J Steroid Biochem Mol Biol. 2023, 106355


Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumor samples. Solheim S, Hutchinson SA, Lundanes E, Wilson SR, Thorne JL, and Roberg-Larsen H. J Steroid Biochem Mol Bio. 2019, 105309


Mass spectrometric detection of 27-hydroxycholesterol in breast cancer exosomes. Roberg-Larsen H, Lund K, Seterdal KE, Solheim S, Vehus T, Solberg N, Krauss S, Lundanes E, and Wilson SR. J Steroid Biochem Mol Biol. 2017, 169:22-28


Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols. Roberg-Larsen H, Lund K, Vehus T, Solberg N, Vesterdal C, Misaghian D, Olsen PA, Krauss S, Wilson SR, and Lundanes E. J Lipid Res. 2014, 55(7):1531-6


Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins. Vehus T, Roberg-Larsen H, Waaler J, Aslaksen S, Krauss S, Wilson SR, and Lundanes E. Sci. Rep. 2016 6: 37507.

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