Group: Lipidomics, University Hospital Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Regensburg, Germany
Key members: Gerhard Liebisch, Silke Matysik, Sabrina Krautbauer
Contact: Silke Matysik,
Research activities: Our research is focused on the development of novel methods to quantify lipid species (fatty acids, glycerophospholipids, sphingolipids, steroids and bile) and their application in clinical studies and basic research.
We use mass spectrometry (MS) for direct flow injection analysis, coupled to liquid (LC) and gas chromatography (GC). Both low and high mass resolution is applied to analyze the lipid composition of human plasma, lipoprotein fractions, faeces, cells and tissues. Beside quantification of lipid species we apply stable isotope-labelled tracer to investigate the dynamics of the lipid metabolism.
Key papers related to ENOR:
Quantification of steroid hormones in human serum by liquid chromatography-high resolution tandem mass spectrometry. Matysik S, Liebisch G. J Chromatogr A. 2017 Dec 1;1526:112-118
Quantification of oxysterols in human plasma and red blood cells by liquid chromatography high-resolution tandem mass spectrometry. Pataj Z, Liebisch G, Schmitz G, Matysik S. J Chromatogr A. 2016 Mar 25;1439:82-8
Relevance in the Use of Appropriate Internal Standards for Accurate Quantification Using LC-MS/MS: Tauro-Conjugated Bile Acids as an Example. Krautbauer S, Büchler C, Liebisch G. Anal Chem. 2016 Nov 15;88(22):10957-10961.