Group: School of Life and Health Sciences, Aston University, Birmingham, UK
Key members: Irundika Dias
Research activities: The primary focus of my research is to investigate the role of lipid oxidation in chronic diseases. In particular, I am investigating oxysterol involvement in Alzheimer’s disease and chronic kidney disease. My work has shown that oxysterol -treated neuronal cell viability was decreased, associated with a decrease in redox potential and an increase amyloid beta secretion through lipid raft formation. I developed a simple blood-brain barrier model to investigate the oxysterol mobility. Recently, I have developed a high sensitive targeted liquid chromatography and mass spectrometry (LC-MS/MS) based multiple reaction monitoring (MRM) method to quantify oxysterols in cells, tissue and biological fluids.
Key papers related to ENOR:
1. Hypercholesterolaemia-induced oxidative stress at the blood-brain barrier. Dias IH, Polidori MC, Griffiths HR. Biochem Soc Trans. 2014 Aug;42(4):1001-5.
2. Oxidized LDL lipids increase β-amyloid production by SH-SY5Y cells through glutathione depletion and lipid raft formation. Dias IH, Mistry J, Fell S, Reis A, Spickett CM, Polidori MC, Lip GY, Griffiths HR. (2014) Free Radic Biol Med. 2014 Jul 15;75:48-59